This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human IL-6. Samples (orStandards) are added to the micro ELISA plate wells and combined with the specificantibody. Then a biotinylated detection antibody specific for Human IL-6 and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate welland incubated. Free components are washed away. The substrate solution is added to eachwell. Only those wells that contain Human IL-6, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction isterminated by the addition of stop solution and the color turns yellow. The optical density(OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value isproportional to the concentration of Human IL-6. You can calculate the concentration ofHuman IL-6 in the samples by comparing the OD of the samples to the standard curve.

Elabscience^® stands at the forefront of biotechnology innovation, expertly combining independent design, R&D, manufacturing, and sales to deliver premier reagents and services for cell detection research. Our diverse product portfolio includes advanced solutions for detecting membrane and intracellular proteins (Flow cytometry antibodies), secreted proteins (ELISA kits), cell glycolipid metabolic intermediates and inorganic salts (Metabolism Assays), and comprehensive assessments of cellular function and health (Cell Apoptosis Assay, Cell cycle Assay, Cell Proliferation /Cytotoxicity/Viability).

To keep pace with the rapid advancements in research, we are dedicated to the continuous development of cutting-edge antibody and protein reagents, ensuring that our products evolve to meet the latest scientific needs. Our commitment extends beyond cell detection to include sophisticated cell isolation and characterization, empowering researchers to tackle the most complex challenges in cell biology. We pride ourselves on maintaining stringent quality control for every product, enhancing the accuracy and reliability of your experimental results. Our relentless pursuit of excellence since 2009 has established our presence in over 150 countries and regions worldwide.

Elabscience^® is dedicated to addressing the evolving challenges in life sciences and healthcare. With a focus on delivering competitive, innovative solutions and driven by an unwavering commitment to excellence, we strive to be your trusted research partner in life sciences and medicine.

R&D and Production strengths:

ØIndependent R&D and production of core raw materials

Ø160,000  ft²+ Independent R&D lab & production workshops

Ø20,000+ SCI citations